Ovary Under the Microscope (top section)

Ovary (top section), Homo sapiens

Ovary  (top section), prepared microscope slide

This is a frozen section of human ovary tissue stained with H&E. It mostly shows the ovary's supporting tissue (stroma) and blood vessels.

Frozen section of ovary. The section is dominated by densely collagenized ovarian stroma: whorled fascicles of spindle-shaped stromal cells within an eosinophilic collagen matrix, the fibrotic cortical pattern typical of the postmenopausal ovary. Prominent congested medullary blood vessels track through the deeper stroma, and scattered basophilic cellular foci punctuate the fibrous background. No developing follicles are conspicuous at scanning power. Cryosectioning has produced characteristic tissue tearing and fragmentation along the stromal planes, a useful example of frozen-section artifact in a collagen-rich organ.

What to look for

At each magnification

Low power (about 4x)

A section of dense pink and purple tissue with some gaps and tears.

Medium power (about 10x)

Swirls of stromal cells and the blood vessels between them.

High power (about 40x)

The long, thin nuclei of stromal cells and blood cells inside vessels.

Questions and answers

What does ovary tissue look like under a microscope?

Ovary tissue has a dense background of spindle-shaped cells and collagen called stroma, with blood vessels running through it. In younger ovaries, you may also see round follicles, each holding an egg cell.

Why are there no follicles on some ovary slides?

The number of follicles drops with age, and after menopause the ovary is mostly stroma. Any single section is also a very thin slice, so it may miss the follicles that are present.

Based upon data generated by the TCGA Research Network (https://www.cancer.gov/tcga) and made available through the NCI Genomic Data Commons (GDC). See credits.

Category
Histology and Tissue
Scan magnification
20x
Stain
H&E
Tags
normal ovary, ovarian stroma, ovarian cortex, stromal spindle cells, collagen, medullary vessels

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